Figure 2.

PHB inhibits breast cancer cell proliferation, induces cell cycle arrest and promotes cell apoptosis. (A, B) Cell viability was measured by MTT assay 24, 48, or 72 h following transfection of plasmids encoding PHB into MCF-7 and T47D cell lines. (C, D) The MCF-7 and T47D cells were transfected PHB vector or control vector for 48 h, and then cell cycle distribution was determined by flow cytometry analysis using PI staining. (E, F) Apoptosis was detected by flow cytometry analysis following transfection of plasmids encoding PHB into MCF-7 and T47D cell lines. Data were represented as the average of 3 independent experiments. *P < 0.05, as compared with control vector transfected cells.