Figure 4.

CPEB2 is the direct target of miR-181a to modulate the sensitivity of paclitaxel in NPC cells. (A) 293T cells were co-transfected with either 100 nM miR-181a mimcs or control oligos (NC) and 200ng plasimed carrying either wild or mutant 3′-UTR of CPEB2. The relative firefly luciferase activity normalized with Renilla luciferase and was measured 48 h later. (B) The protein level of CPEB2 was examined in CNE1 and CNE2 cells, which were treated with paclitaxel of paclitaxel plus CPEB2. (C) MTT assays were performed for examining the cell viability of CNE1 cells, which were treated with Taxol or Taxol plus CPEB2. (D) Quantification of apoptotic CNE1 cells were performed by flow cytometry analysis. All data was shown as mean ± s.e.m. from 3 independent experiments. * represents p < 0.05, ** represents p < 0.01 and *** represents p < 0.001.