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. 2017 Feb 22;16(8):765–775. doi: 10.1080/15384101.2017.1295186

Figure 5.

Figure 5.

The abundance of H3K27me3 and H3K36me3 in MRI-classified GBM. (A) Western blot detected slightly elevated levels of H3K27me3 in MRI-classified SVZ-associated group I and group II GBM compared with control regions. (B) The level of H3K36me3 is increased in histone fractions of GBM patients. The unmodified H4 was used for loading control. The comparison was performed using normal brain specimens obtained from temporal, frontal, and parietal lobes. Quantification of enrichment ratio was done by ImageJ software. Enrichment ratio reflects intensity of tumor relative to mean of temporal, frontal, and parietal lobes after normalization to H4 loading control. The MRI-classified group I GBM is associated with both SVZ and cortex (SVZ+ cortex+), group II GBM (SVZ+ cortex−), group III GBM (SVZ− cortex+), group IV GBM (SVZ− cortex−). The gliosis associated with temporal region and gliomas associated with hippocampus were analyzed in parallel.