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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Trauma Acute Care Surg. 2017 May;82(5):853–860. doi: 10.1097/TA.0000000000001413

Figure 3. Effects of mtDAMPs and external PMN on P. aeruginosa clearance (PA) in lung.

Figure 3

CD-1 mice were separated into three groups. 1. Saline/PA: Saline i.p. injection (time 0) followed by P. aeruginosa (24 h) i.t. 2. MTD/PA: MTD i.p. (time 0) followed by P. aeruginosa i.t. (24 h), 3. MTD/PA/PMN: MTD i.p. (time 0) followed by P. aeruginosa i.t. (24 h) and BM-PMN i.t. (27 h). Animals were sacrificed t=48 h. Lung homogenates were prepared to determine bacterial presence. MTD were prepared from 10% of total liver in saline. 50 μL of OD600=0.1, P. aeruginosa was injected intratracheally.

BM-PMN were freshly prepared from donor CD-1 mice and ~2×106 cells were injected intratracheally. Saline/Bac: N=10, MTD/Bac: N=11, MTD/Bac/PMN: N=12. Mean and SD values are shown.

* denotes a significant difference by One-Way ANOVA, Tukey.