Fig. 5.

AUC sedimentation profile of Dox-NP™ suspension in A) sucrose solution with density of 1.05 g/mL, B) sucrose solution with density of 1.06 g/mL. Y-axis is optical absorption at 490 nm while the X axis represents radial positions (distance in cm from center of rotation) in the sample cell. The changing colour of the curves from dark blue to red corresponds to increasing centrifugation time. C) Mass based size distribution of Dox-NP™ (solid line) and empty vesicles (dashed line) calculated from AUC measurement using interference optics. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)