Fig. 6.

Distribution of theDrosophilaCTPsyn wild-type and mutant proteins in cells co-expressing the constructs. Wild-type CTPsyn-mCherry (A′-E′) was transfected along with Venus-tagged CTPsyn mutant proteins CTPsyn^Δ20 (A), CTPsyn^Κ2Ε (B), CTPsyn^Y3E (C), ^pyrG/1−22CTPsyn (D) and ^pyrG/N4KCTPsyn (E), and their distribution in the cytoplasm monitored. While CTPsyn^Δ20, CTPsyn^Κ2Ε , CTPsyn^Y3E and ^pyrG/1−22CTPsyn proteins assembled into aggregates that did not overlap with CTPsyn cytoophidia (A′′-D′′), ^pyrG/N4KCTPsyn co-assembled with the cytoophidia (E′′). DNA staining with Hoescht is shown in blue and the Venus- and mCherry-tagged proteins are in green and red, respectively. Scale bar=5 µm.