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. Author manuscript; available in PMC: 2017 Apr 26.
Published in final edited form as: Nat Struct Mol Biol. 2016 Feb 29;23(4):278–285. doi: 10.1038/nsmb.3182

Figure 3. Nascent chains of FLN5 emerging from the ribosome monitored by NMR spectroscopy.

Figure 3

(a) 1H-13C correlation spectra of [U-2H; Ileδ1-13CH3 labeled] FLN5 RNCs (black), isolated, natively folded FLN5 (cyan) and isolated unfolded FLN5∆16 (orange). Resonances marked “R” arise from background labeling of 70S ribosomal proteins27. (b) 1H-15N correlation spectra of U-15N-labelled FLN5 RNCs, isolated FLN5∆12 (blue), and unfolded FLN5-6∆18 (green). Resonances used for the analysis of unfolded conformations are labeled in the FLN5+21 RNC spectrum.