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. 2017 Apr 26;12(4):e0175507. doi: 10.1371/journal.pone.0175507

Fig 2. AMPKα knockdown abolishes antagomiR-451-medaitd HUVEC protection again OGD.

Fig 2

Parental HUVECs (“Parental”) as well as HUVECs expressing antagomiR-451 or antagomiR-C were maintained under OGD for 3 hours, followed by 24 hours of re-oxygenation, p-/total AMPKα and ACC were shown, blot data were quantified (A).HUVECs, with/outantagomiR-451, were infected with lentiviral scramble control shRNA (“SCR shRNA”) or AMPKα shRNA (“s1/s2”); Cells were then subjected to the OGD for 3 hours, followed by 24 hours of re-oxygenation; Expressions of p-/total AMPKα and ACC were shown, blot data were quantified (B); Cell survival and necrosis were tested by MTT assay (C) and LDH release assay (D), respectively. Bars indicate mean ± standard deviation (SD, n = 5).*p<0.05 vs.“Parental” cells (A).*p<0.05 vs. group “SCR shRNA” (B).*p<0.05 vs. group “Mock” (C and D). #p<0.05 vs.“SCR shRNA” group(C and D).Each experiment was repeated four times and similar results were obtained.