Skip to main content
. 2017 Apr 26;12(4):e0175507. doi: 10.1371/journal.pone.0175507

Fig 5. AntagomiR-451 attenuates OGD-induced programmed necrosis in HUVECs.

Fig 5

HUVECs expressing antagomiR-451 or antagomiR-C were maintained under OGD for 3 hours, followed by re-oxygenation for indicated time, mitochondrial fraction was isolated, and the association between Cyp-D and p53 was tested by the co-immunoprecipitation assay (“Mito-IP”) assay (A, left panel). “Input” tested Cyp-D and p53 expression (A, right panel);Mitochondrial depolarization was tested by JC-10 assay (B); Relative ROS content (C) and lipid peroxidation level (D) were also tested. Bars indicate mean ± standard deviation (SD, n = 5).*p<0.05 vs. group “Mock”. #p<0.05 vs. “OGD” of “antagomiR-C” cells. Each experiment was repeated three times and similar results were obtained.