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. 2017 Apr 26;12(4):e0176236. doi: 10.1371/journal.pone.0176236

Table 1. List of marker used for blast resistance genes.

Sl.No. R Gene Chr no. Marker used Positive control Primer sequence Type of marker Annealing temp. Expected size (bp) References
Forward (5`-3`) Reverse (5`-3`)
1. Pib 2 Pb28 IRBLB-b GACTCGGTCGACCAATTCGCC ATCAGGCCAGGCCAGATTTG SNP 60 388 [30]
2. Piz 6 Z56592 IRBLZ-Fu GGACCCGCGTTTTCCACGTGTAA AGGAATCTATTGCTAAGCATGAC SNP 60 292 [30]
3. Piz-t 6 Zt56591 IRBLZT-t TTGCTGAGCCATTGTTAAACA ATCTCTTCATATATATGAAGGCCAC SNP 60 257 [30]
4. Pik 11 K39512 IRBLZK-Ka GCCACATCAATGGCTACAACGTT CCAGAATTTACAGGCTCTGG SNP 60 112 [30]
5. Pik-p 11 K3957 IRBLZKP-k60 ATAGTTGAATGTATGGAATGGAAT CTGCGCCAAGCAATAAAGTC SNP 60 148 [30]
6. Pik-h 11 Candidate gene marker IRBLKH-k3 CATGAGTTCCATTTACTATTCCTC ACATTGGTAGTAGTGCAATGTCA Gene based marker 55 1500 [38]
7. Pi9 6 195R-1 IRBL9-w ATGGTCCTTTATCTTTATTG TTGCTCCATCTCCTCTGTT STS 56 2,000 [45]
9-Pro TGATTATGTTTTTTATGTGGGG ATTAGTGAGATCCATTGTTCC Allele specific 50 111 for Pi2/Piz-t allele,
128 for Pi9 allele
[46]
Pi9-i GCTGTGCTCCAAATGAGGAT GCGATCTCACATCCTTTGCT FNP 54 [47]
8. Pi2 6 Pi-2GM IRBLZ5-CA GATTTAGTTCAGGAAAACACTC TGGAAGCCTCATTGATCATC Gene specific 55 2344 [31]
2-LRR CGTTGTATAGGACAGTTTCATT AATCTAGGCACTCAAGTGTTC Allele specific 50 436/439 [46]
Pi2-i CAGCGATGGTATGAGCACAA CGTTCCTATACTGCCACATCG FNP 52 450/282 [47]
9. Pita/Pita-2 12 Pita3 IRBLTA-KI AGTCGTGCGATGCGAGGACAGAAAC GCATTCTCCAACCCTTTTGCATGCAT SNP 59 861 [30]
YL155/YL87 - AGCAGGTTATAAGCTAGGCC CTACCAACAAGTTCATCAAA Dominant 55 1042 [48,49]
10. Pi1 11 RM1233 C101LAC GTGTAAATCATGGGCACGTG AGATTGGCTCCTGAAGAAGG SSR 55 170/155 [18]
11. Pi5 9 40N23r IRBLz5-M TGTGAGGCAACAATGCCTATTGCG CTATGAGTTCACTATGTGGAGGCT InDel 55 700/480 [50]
12. Pikm 11 k2167 IRBLKMTS CGTGCTGTCGCCTGAATCTG CACGAACAAGAGTGTGTCGG InDel 55 619/300 [51]