Table 1. List of marker used for blast resistance genes.
Sl.No. | R Gene | Chr no. | Marker used | Positive control | Primer sequence | Type of marker | Annealing temp. | Expected size (bp) | References | |
---|---|---|---|---|---|---|---|---|---|---|
Forward (5`-3`) | Reverse (5`-3`) | |||||||||
1. | Pib | 2 | Pb28 | IRBLB-b | GACTCGGTCGACCAATTCGCC | ATCAGGCCAGGCCAGATTTG | SNP | 60 | 388 | [30] |
2. | Piz | 6 | Z56592 | IRBLZ-Fu | GGACCCGCGTTTTCCACGTGTAA | AGGAATCTATTGCTAAGCATGAC | SNP | 60 | 292 | [30] |
3. | Piz-t | 6 | Zt56591 | IRBLZT-t | TTGCTGAGCCATTGTTAAACA | ATCTCTTCATATATATGAAGGCCAC | SNP | 60 | 257 | [30] |
4. | Pik | 11 | K39512 | IRBLZK-Ka | GCCACATCAATGGCTACAACGTT | CCAGAATTTACAGGCTCTGG | SNP | 60 | 112 | [30] |
5. | Pik-p | 11 | K3957 | IRBLZKP-k60 | ATAGTTGAATGTATGGAATGGAAT | CTGCGCCAAGCAATAAAGTC | SNP | 60 | 148 | [30] |
6. | Pik-h | 11 | Candidate gene marker | IRBLKH-k3 | CATGAGTTCCATTTACTATTCCTC | ACATTGGTAGTAGTGCAATGTCA | Gene based marker | 55 | 1500 | [38] |
7. | Pi9 | 6 | 195R-1 | IRBL9-w | ATGGTCCTTTATCTTTATTG | TTGCTCCATCTCCTCTGTT | STS | 56 | 2,000 | [45] |
9-Pro | TGATTATGTTTTTTATGTGGGG | ATTAGTGAGATCCATTGTTCC | Allele specific | 50 | 111 for Pi2/Piz-t allele, 128 for Pi9 allele |
[46] | ||||
Pi9-i | GCTGTGCTCCAAATGAGGAT | GCGATCTCACATCCTTTGCT | FNP | 54 | [47] | |||||
8. | Pi2 | 6 | Pi-2GM | IRBLZ5-CA | GATTTAGTTCAGGAAAACACTC | TGGAAGCCTCATTGATCATC | Gene specific | 55 | 2344 | [31] |
2-LRR | CGTTGTATAGGACAGTTTCATT | AATCTAGGCACTCAAGTGTTC | Allele specific | 50 | 436/439 | [46] | ||||
Pi2-i | CAGCGATGGTATGAGCACAA | CGTTCCTATACTGCCACATCG | FNP | 52 | 450/282 | [47] | ||||
9. | Pita/Pita-2 | 12 | Pita3 | IRBLTA-KI | AGTCGTGCGATGCGAGGACAGAAAC | GCATTCTCCAACCCTTTTGCATGCAT | SNP | 59 | 861 | [30] |
YL155/YL87 | - | AGCAGGTTATAAGCTAGGCC | CTACCAACAAGTTCATCAAA | Dominant | 55 | 1042 | [48,49] | |||
10. | Pi1 | 11 | RM1233 | C101LAC | GTGTAAATCATGGGCACGTG | AGATTGGCTCCTGAAGAAGG | SSR | 55 | 170/155 | [18] |
11. | Pi5 | 9 | 40N23r | IRBLz5-M | TGTGAGGCAACAATGCCTATTGCG | CTATGAGTTCACTATGTGGAGGCT | InDel | 55 | 700/480 | [50] |
12. | Pikm | 11 | k2167 | IRBLKMTS | CGTGCTGTCGCCTGAATCTG | CACGAACAAGAGTGTGTCGG | InDel | 55 | 619/300 | [51] |