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Colorimetric TUNEL assay to detect the induction of apoptosis in tumor sections from different treatments. Tumors from animals treated once a day with 80 mg/kg metformin (MET) (A), 50 mg/kg curcumin (CUR) (B), combination of both (C) and a vehicle (D). Apoptotic nuclei are stained dark brown. Four mice were examined in each treatment.
