Fig 1. IEC-1 is required for normal circadian clock function.

(A) Race tube assays of the wild-type and iec-1^KO strains. (B) Amino acid sequence alignment of the zf-C2H2 domains of IEC-1 from Neurospora crassa, Penicillium brasilianum, Aspergillus fumigates and Nectria haematococca. (C) Race tube assays of the wild-type strain, iec-1^KO strain, and iec-1^KO, qa-Myc-IEC-1 transformants in a race tube with or without QA. Growth media on the race tubes did not consist of glucose. (D) Luciferase reporter assay showing the frq promoter activity in the wt, frq-luc and iec-1^KO, frq-luc strains grown in DD for several days. Raw data were normalized to subtract the baseline calculated by the LumiCycle analysis software.