Fig 5. The establishment of nucleosomal barriers at the frq promoter by the INO80 complex prevents RNA pol II initiation.

(A) ChIP analysis showing H3 density at the C-box, TSS or ORF middle regions of the frq locus in the wild-type, ies-1^KO, and ino80^KO strains. The strains were grown in 2% glucose liquid media. Significance was assessed by two-tailed t-test. *P<0.05, **P<0.01. Error bars show the mean ±S.D. (n = 3). (B) ChIP analysis showing the recruitment of SET-2 and enrichment of H3K36me3 at the ORF 3’ of frq in the wild-type and ino80^KO strains. The strains were grown in 2% glucose liquid media. Significance was assessed by two-tailed t-test. *P<0.05, **P<0.01. Error bars show the mean ±S.D. (n = 3).