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. 2017 Apr 26;12(4):e0176382. doi: 10.1371/journal.pone.0176382

Fig 1. Depletion of SF3B1 inhibits the HSR.

Fig 1

(A) qRT-PCR analysis of mRNA levels of two heat shock genes, HSPA6 and DNAJB1, in cells that have been treated with either HSF1 siRNA or SF3B1 siRNA and subjected to a 1 hour heat shock at 42°. Data is shown relative to mRNA levels in heat shocked cells treated with a nonsilencing control siRNA. (B) Western blot analysis of cells treated with SF3B1 siRNA or a nonsilencing control siRNA probed with both anti-SF3B1 and anti-ACTIN antibodies. Two biologically independent replicates for each sample are shown. (C) Western blot analysis of cells treated with SF3B1 siRNA or a nonsilencing control siRNA and probed with anti-HSP70 or anti-ACTIN antibodies. Heat shocked cells were subjected to a 1 hour at 42° and then allowed to recover for 4 hours at 37° before harvesting. Induction levels of HSP70 were quantitated by densitometry from the blots and normalized to ACTIN. Fold induction upon heat shock is shown. (D) Diagram showing the qPCR primer locations for HSPA6 and DNAJB1.