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. 2017 Apr 26;12(4):e0176382. doi: 10.1371/journal.pone.0176382

Fig 4. Inhibition of SF3B1 affects HSF1 transcriptional activation.

Fig 4

Quantitation of relative luminescence from cells expressing an HSF1-Gal4 chimera containing the HSF1 activation domain and the Gal4 DNA-binding domain exposed to varying levels of Pladienolide B. Luciferase activity was monitored from a firefly luciferase reporter with a Gal4 binding element that was co-transfected with the chimera. Luciferase activity was normalized to the no drug control and a VP16-Gal4 control. Dual luciferase with a general NanoLuc reporter was also measured to correct for differences in cell density and transfection efficiency. * indicates p-value < 0.05.