Figure 3.
Expression of phototransduction proteins in the hazy mutant retina. Head sections were taken of control and hazyhazy flies, and stained with antibodies against different phototransduction proteins (green) and with Hoechst (used to label cell nuclei, magenta). (A-P) One group of flies was dissected on the day they eclosed. At this age we did not detect neither Rh6 (I) nor Trpl (J) in the retina of hazy mutants, but most of the phototransduction proteins that we tested were correctly expressed: Rh1 (K), Gαq (L), NorpA (M), Trp (N), InaD (O) and Arr1 (P). (a-p) A second group of flies were dissected 10 d after eclosion. Neither Rh6 (i) nor Trpl (j) were expressed in the retina of these older hazy mutants, and most phototransduction proteins showed decreased expression levels: Rh1 (k), Gαq (l), NorpA (m), Trp (n) and InaD (o). Only Arr1 (p) expression did not seem reduced over time in the hazy mutant retina. Scale bars represent 50 µm.