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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Protein Expr Purif. 2016 Nov 17;131:109–118. doi: 10.1016/j.pep.2016.11.006

Fig. 2.

Fig. 2

Expression and activity of CB2.

A, Western blot performed on E. coli membranes expressing CB2. Proteins were probed with rabbit polyclonal antibody against His5 tag.

B, activation rates of G protein on E. coli membrane expressing CB2. The receptor was activated by addition of 10 μM agonist CP-55,940 and the rates of activation of G protein analysed by measuring the accumulation of 35S-γ-GTP-Gαi1 complex as described previously.(5)