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. 2017 Apr 25;112(8):1654–1662. doi: 10.1016/j.bpj.2017.02.040

Figure 3.

Figure 3

Forces of tether formation, extension, and holding in control and cells treated with CD and latrunculin to disrupt actin filaments. (A and B) Sample traces show bead positions in the optical trap during membrane tether formation (the peak), extension (after the peak to 10 s), and holding (10 to 15 s) for a control DRG neuron (A) and a DRG neuron after 30 min treatment with 10 μM CD (B). The axis on the right side of (A) or (B) shows the corresponding tether forces converted from the bead position shift. (C) Sample images show actin staining by phalloidin in DRG neurons in control (upper panel) and after 30 min treatment with 10 μM CD (lower panel). (D–F) Summary data of the tether forces during membrane tether formation (D, the values at the peaks), extension (E, the values between 5 to 10 s), and holding (F, the values between 10 to 15 s). In (D)–(F), black bars are the control group, blue bars are cells after the treatment with 10 μM CD, and red bars are cells after the treatment with 10 μM latrunculin. Data represent mean ± SE, ∗p < 0.05; ∗∗p < 0.01.