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. 2017 Apr 25;112(8):1654–1662. doi: 10.1016/j.bpj.2017.02.040

Figure 4.

Figure 4

Forces of tether formation, extension, and holding in control and cells treated with methyl-β-cyclodextrin to sequester membrane cholesterol. (A and B) Sample traces show bead positions in the optical trap during membrane tether formation (arrow indicated), extension, and holding for a control DRG neuron (A) and a DRG neuron after 30 min treatment with 10 mM MβCD (B). The axis on the right side of (A) or (B) shows the corresponding tether forces converted from the bead position shift. (C–E) Summary data of the tether forces during membrane tether formation (C), extension (D), and holding (E). In (C)–(E), black bars are the control group, dotted bars are cells after treatment with 3 mM MβCD, and shaded bars are cells after the treatment with 10 mM MβCD. Data represent mean ± SE, ∗p < 0.05; ∗∗p < 0.01.