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(A) FRAP bleach regions are numbered according to their location: the perinuclear cup (Region 1), the upper vegetal cytoplasm (Region 2), and the lower vegetal cytoplasm (Region 3). (B) Shown is a representative oocyte in which a 5 μm circular ROI of VLE-MS2 RNA bound by MCP-mCh was bleached in the vegetal cytoplasm (Region 3). Scale bars represent 10 μm. To see this figure in color, go online.
