Skip to main content
. 2017 Apr 6;174(10):1131–1146. doi: 10.1111/bph.13765

Figure 3.

Figure 3

The combination treatment of MK‐2206 and EF24 induces apoptosis in human gastric cancer cells. (A) The effects of combined MK‐2206 and EF24 treatment on the viability of human gastric cancer cells. Cells were treated with increasing concentrations of MK‐2206 and 2 μM EF24 for 24 h. (B) The induction of apoptosis as assessed by Annexin V/PI staining following combined treatment with MK‐2206 and EF24 for 24 h. (C) Western blot analysis of apoptosis‐related proteins in cells treated with MK‐2206 and EF24 for 16 h. Figure showing levels of Bcl‐2, Bax, cleaved‐PARP and cleaved‐caspase 3. (D) Effect of combined treatment on colony formation. Cells were treated for 24 h and stained with crystal violet on day 8. (E) Gastric cancer cells were pretreated with 5 mM NAC for 2 h before exposure to MK‐2206 and EF24 for 24 h. Apoptosis was detected by Annexin V/PI staining. (F) Quantification of data presented in panel (E). Data are reported as mean ± SEM and analysed by Student's t‐test; n = 5 independent experiments; *P < 0.05. (G) Analysis of cleaved‐PARP, Bcl‐2 and Bax levels in SGC‐7901 cells pretreated with NAC. All representative images are from five independent experiments.