Fig. 1.

Dedifferentiated fat cells were isolated as follows: Fat tissue fragments were minced using scissors (1), and were digested in 0.12% collagenase type I medium (2). The resulting material was centrifuged (3). The floating fraction was placed in an inverted culture flask that was completely filled with culture medium (4). After 7 days, the flask was reversed (5). Adipose-derived stem cells were isolated the same as dedifferentiated fat cells (1, 2, and 3). The stromal vascular fraction was filtered through a 100-µm mesh and centrifuged and then resuspended in a culture medium (6).