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. 2017 Apr 25;112(8):1692–1702. doi: 10.1016/j.bpj.2017.03.012

Figure 3.

Figure 3

Quantification of cluster spread for rAb bound over M1- and M23-AQP4. (A–C) Representative antibody images are shown for several AQP4 autoantibodies binding to M23-AQP4. An intracellular antibody (left) detects all AQP4; extracellular autoantibody binding (middle) is shown with pixel intensity traces (right) for all rows/columns labeled with an arrow. (D) Calculated mean FWHMrAb spread across an entire image. Data was compared using one-way ANOVA with Tukey’s test for multiple comparisons (∗, adjusted p < 0.05). STED image scale: 19.5 × 19.5 nm/pixel. Scale: all image pixels are 19.5 × 19.5 nm.