Figure 1.
Guinier plots for phosphorylated (A) and unphosphorylated (B) rOPN. Rg and I0 were recovered from the fits of the straight lines where (q × Rg) ≤ 1.3. (C) Expected Rg values for chemically denatured proteins were determined using Eq. 3, where the region between dashed lines represents the confidence interval of the Flory equation. The diamonds show experimental Rg obtained for phosphorylated (darker) and unphosphorylated rOPN (lighter). The lower continuous gray line shows the expected Rg value for globular proteins. (D) P(r) distributions of phosphorylated (black) and unphosphorylated (gray) rOPN, from which the Rg and Dmax were obtained. (E) Kratky plots are shown (same color scheme as D).