Figure 4.

STICCS analysis of two-channel TIRF-SIM data. (a) Jurkat T cells were imaged using two-color TIRF-SIM with F-actin labeled with EGFP-LifeAct and α-actinin labeled with mCherry (middle two images) and cross-correlation outputs (right). Scale bars, 5 μm. (b) Cross-correlation heatmaps from a toroidal ROI at the dSMAC of the cell of velocities (left) and directionality (right) are shown, as described in Fig. 3. (c) Scatter plots of whole cell mean velocities (left = 0.78 ± 0.19) and directionalities (right = 0.92 ± 0.07) are given. (d) Given here is a radial profile plot of single cell, showing normalized fluorescent intensity of actin and α-actinin distribution versus distance from cell center (n = 19). To see this figure in color, go online.