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. 2016 Jul 13;174(10):1077–1089. doi: 10.1111/bph.13522

Figure 1.

Figure 1

Concentration–response relationships for activation of ERK1/2, Akt, p38MAPK and S6RP and inhibition of cAMP production by hINSL5 and mINSL5 in CHO‐RXFP4 cells. In (A) phosphorylation of ERK1/2 (5 min; n = 6), (B) Akt Ser473 (5 min; n = 6), (C) Akt Thr308 (5 min; n = 6), (D) p38MAPK (15 min; n = 5) and (E) S6RP (30 min; n = 5), with in (F) inhibition of forskolin‐stimulated cAMP accumulation [30 min; n = 5 (mINSL5), n = 7 (hINSL5)]. CHO‐RXFP4 cells were treated with increasing concentrations of hINSL5 or mINSL5 (10−11 to 10–6.5 M). Results are normalized to the maximum response to hINSL5 in protein phosphorylation assays or % of response to 3 μM forskolin in cAMP accumulation assay. Data points represent mean ± SEM of independent experiments. Veh, vehicle.