Figure 2.

DHI significantly inhibited the expression of genes involved in hepatic fibrosis in BDL rat livers. (A) mRNA expressions of Col1a1, Mmp‐2, Acta2, Timp1/2 and Tgfβ1 in the sham, BDL‐NS and BDL‐DHI livers were normalized against Gapdh. (B) Western blot analysis of α‐SMA, collagen 1a1 (COL1A1) and TGFβ1 expressions in rat liver samples. GAPDH served as the loading control. (C) Western blot results for each animal are quantified in the three groups. The data are expressed as the mean ± SEM (n = 6 in sham group; n = 7 in BDL‐NS/BDL‐DHI group), #P < 0.05; significantly different from sham group, *P < 0.05; significantly different from BDL‐NS group ANOVA followed by Tukey's test.