REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-His	QIAGEN	Cat# 34660
Rabbit polyclonal anti-DAF-2	This Study	N/A
Rabbit polyclonal anti-CHN-1	This Study	N/A
Mouse monoclonal anti-FLAG	Sigma-Aldrich	Cat# F3165; RRID: AB_259529
Rabbit anti-UNC-45	Gazda et al., 2013	N/A
Mouse Monoclonal anti-alpha Tubulin (Clone DM 1A)	Sigma-Aldrich	Cat# T9026; RRID: AB_477593
Mouse Monoclonal anti-alpha Tubulin (Clone B-5-1-2)	Sigma-Aldrich	Cat# T6074; RRID: AB_477582
Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG+IgM	Jackson Immuno Research	Cat# 115-035-003; RRID: AB_10015289
Peroxidase-conjugated AffiniPure Mouse Anti-Rabbit IgG+IgM	Jackson Immuno Research	Cat# 211-035-109; RRID: AB_2339150
Donkey anti-rabbit IRDye 800CW/680	Li-Cor	Cat# 926-32223; RRID: AB_621845
Donkey anti-mouse IRDye 800CW/680	Li-Cor	Cat# 926-32212; RRID: AB_621847
Mouse monoclonal anti-Actin	Abcam	Cat# ab8224; RRID: AB_449644
Rabbit polyclonal anti-AKT	Cell Signaling	Cat# 9272; RRID:AB_329827
Rabbit monoclonal anti-Phospho AKT (Ser473)	Cell Signaling	Cat# 4060S; RRID: AB_2315049
Rabbit polyclonal anti-phospho Drosophila AKT (Ser505)	Cell Signaling	Cat# 4054S; RRID: AB_331414
Rabbit monoclonal anti-ATG7	Novus Biologicals	Cat# NBP1-95872; RRID: AB_11011190
Rabbit polyclonal anti-Chicken IgG	Sigma-Aldrich	Cat# C2288; RRID: AB_258791
Rabbit polyclonal anit-CHIP	Milipore	Cat# PC711; RRID: AB_2198058
Rat monoclonal anti-GFP	Chromotek	Cat# 3h9, ABIN398304; RRID: AB_10773374
Mouse monoclonal anti-Histidine-Tag	Serotec	Cat# MCA1396; RRID: AB_322084
Rat monoclonal anti-Hsp90	Abcam	Cat# ab13494; RRID: AB_300398
Rabbit polyclonal anti-LC3B	Novus Biologicals	Cat# NB100-2220; RRID: AB_10003146
Mouse monoclonal anti-p62	Santa Cruz	Cat# sc-28359; RRID: AB_628279
Rabbit anti-PDK1	Cell Signaling	Cat# 3062; RRID: AB_2236832
Rabbit polyclonal anti-mTOR	Abcam	Cat# ab45989; RRID: AB_944296
Rabbit monoclonal anti-IGF1R	Cell Signaling	Cat# 9750S; RRID: AB_10950969
Rabbit polyclonal anti-INSR	Santa Cruz Biotechnology	Cat# sc-711; RRID: AB_631835
Goat polyclonal anti-gamma Tubulin	Santa Cruz Biotechnology	Cat# sc-7396; RRID: AB_2211262
Rabbit polyclonal anti-Stub1 (CHIP)	Abcam	Cat# 69451; RRID: AB_2198071
Rabbit polyclonal anti-dINSR	This Study	N/A
Bacterial and Virus Strains
BL21-CodonPlus (DE3)-RIL	New England Biolabs	Cat# C2530H
OP50	CGC	N/A
HT115	CGC	N/A
Chemicals, Peptides, and Recombinant Proteins
completeTablets EDTA free	Sigma-Aldrich	Cat# 4693132001
UbcH5b	Boston Biochem	Cat# E2-622
FLAG::Ubiquitin	Boston Biochem	Cat# U-120
E1 activating Enzyme	Enzo Life Sciences	Cat# BML-UW9410-0050
Ubiquitin Conjugation Reaction Buffer	Enzo Life Sciences	Cat# BML-KW9885-0001
ATP Regeneration Solution	Enzo Life Sciences	Cat# BML-EW9810-0100
Ubiquitin	Sigma-Aldrich	Cat# U6253
UbK0	Boston Biochem	Cat# UM-Nok
FLAG M2 Affinity Gel	Sigma-Aldrich	Cat# A2220
3X FLAG peptide	Sigma-Aldrich	Cat# F4799
Bafilomycin A1	LC-Labs	Cat# B1080
Chloroquine diphosphate salt	Sigma-Aldrich	Cat# C6628
Dynamin Inhibitor V-34-2	Calbiochem	Cat# 324414
Methylviologen dichloride hydrate	Sigma-Aldrich	Cat# 856177-CAS75365-73-0
MG132	Peptanova	Cat# 3175-V
L-Canavanine	Sigma-Aldrich	Cat# C1625
Paraquat dichloride hydrate	Sigma-Aldrich	Cat# 36541
RU486 (Mifepristone)	Sigma-Aldrich	Cat# M8046
VSV::CHN-1::6His	This Study	N/A
VSV::CHN-1 H218Q::6His	This Study	N/A
VSV::CHN-1 ΔU-box::6His	This Study	N/A
VSV: CHN-1 N9D, K13A, K73A::6His	This Study	N/A
UFD-2::6His	This Study	N/A
6His::DAF-2 C terminus (aa 1205-1928)	This Study	N/A
WWP-1::6His	This Study	N/A
CHIP	Arndt et al., 2005	N/A
INSR Full Length	This Study	N/A
INSR K1047R	This Study	N/A
dINSR (aa 1815-2144)	This Study	N/A
INSR C terminus (aa 987-1428)	This Study	N/A
TRIzol	Invitrogen	Cat# 15596026
Critical Commercial Assays
ECL kit	GE Healthcare	Cat# RPN2232
QuickChange Lightning Site-Directed Mutagenesis Kit	Agilent Technologies	Cat# 210519
InviTrap Spin Universal RNA Mini Kit	Stratec	Cat# 1060100300
jetPRIME transfection reagent	Peqlab	Cat# 114-75
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat# 4368814
Brilliant III Ultra-Fast SYBR Green QPCR Master Mix	Agilent Technologies	Cat# 600882
Sso Fast EvaGreen supermix	Bio-Rad	Cat# 1725201
iScript cDNA Synthesis Kit	Bio-Rad	Cat# 1708891
RNeasy Mini Kit	QIAGEN	Cat# 74104
Experimental Models: Cell Lines
HEK293 (Human embryonic kidney cells)	Sigma-Aldrich	Cat# 85120602
HeLa (Epitheloid cervix carcinoma cells (female))	Sigma-Aldrich	Cat# 93021013
Experimental Models: Organisms/Strains
C. elegans: unc-119(ed4)III; hhIs136[unc-119(+); chn-1p::chn-1::FLAG]	This study	N/A
C. elegans:unc-119(ed4)III; hhIs145[unc-119(+); chn-1p::chn-1(U-box)::FLAG]	This study	N/A
C. elegans: Bristol (N2) strain as wild-type (WT)	CGC	N2
C. elegans: chn-1(by155)I	CGC	WormBase ID: WBVar00000641
C. elegans:chn-1(tm2692)I	CGC	WormBase ID: WBVar00251538
C. elegans:daf-16(mgDf47)I	CGC	WormBase ID: WBVar00275574
C. elegans:daf-16(m26)I	CGC	WormBase ID: WBVar00088538
C. elegans:ufd-2(tm1380)II	CGC	WormBase ID: WBVar00250374
C. elegans:daf-2(e1370)III	CGC	WormBase ID: WBVar00143949
C. elegans:daf-2(e1368)III	CGC	WormBase ID: WBVar00143947
C. elegans:daf-2(m212)III	CGC	WormBase ID: DR608
C. elegans:daf-2(m596)III	CGC	WormBase ID: WBVar00088714
C. elegans:daf-2(m579)III	CGC	WormBase ID: WBVar00088710
C. elegans:daf-2(sa193)III	CGC	WormBase ID: WBVar00242513
C. elegans:daf-2(e979)III	CGC	WormBase ID: WBVar00143653
C. elegans:daf-2(e1391)III	CGC	WormBase ID: WBVar00143967
C. elegans:daf-2(m577)III	CGC	WormBase ID: WBVar00088709
C. elegans:daf-2(gk390525)III	CGC	WormBase ID:WBVar00813221
C. elegans:eat-2(ad465)	CGC	WormBase ID: WBVar00000014
C.elegans: unc-119(ed4)III	CGC	WormBase ID: WBVar00145094
C. elegans:dgEx80[pAMS66 vha-6p::Q44::YFP + rol-6(su1006)]	Mohri-Shiomi and Garsin, 2008	N/A
C. elegans:zIs356(daf-16p::daf-16::GFP; rol-6)	Lee et al., 2001	N/A
C. elegans:rmIs133(unc-54::Q40::YFP)	Morley et al., 2002	N/A
D. melanogaster: w1118	Bloomington Stock Center	RRID: BDSC_3605
D. melanogaster: actin-Gal4	Bloomington Stock Center	RRID: BDSC_3954
D. melanogaster: RNAi-dCHIP	VDRC	RRID: FlyBase_FBst0460471
D. melanogaster: TIGS-2	Scott Pletscher	N/A
D. melanogaster: UAS-dCHIP	Verena Arndt	N/A
Oligonucleotides
See Table S3 for primer sequences	This Study	N/A
siRNA human CHIP 5	QIAGEN	Cat# SI00081963
siRNA human CHIP 5	QIAGEN	Cat# SI03109659
siRNA human ATG7 1	QIAGEN	Cat# SI04194883
siRNA human ATG7 3	QIAGEN	Cat# SI04231360
Allstars negative control siRNA	QIAGEN	Cat# SI03650318
Recombinant DNA
pET21a-VSV::CHN-1::6His	This Study	N/A
pET21a- VSV::CHN-1 H218Q::6His	This Study	N/A
pET21a- VSV::CHN-1 ΔU-box::6His	This Study	N/A
pET21a- VSV: CHN-1 N9D, K13A, K73A::6His	This Study	N/A
pET21a-UFD-2::6His	This Study	N/A
pT7-6His::DAF-2 C terminus (aa 1205-1928)	This Study	N/A
pET21a-WWP-1::6His	This Study	N/A
pCMV-tag2b-CHIP	Arndt et al., 2005	N/A
pCMV-tag2b-INSR Full Length	This Study	N/A
pCMV-tag2b-INSR K1047R	This Study	N/A
pETM-11-dINSR (aa 1815-2144)	This Study	N/A
pMAL-C2-INSR C terminus (aa 987-1428)	This Study	N/A
pEGFP-polyQ-25	Westhoff et al., 2005	N/A
pEGFP-polyQ-103	Westhoff et al., 2005	N/A
Software and Algorithms
GraphPad Prism 5	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Image Studio 4.0	LI-COR Biosciences	https://www.licor.com/bio/products/software/image_studio/
Excel 2013	Microsoft	https://products.office.com/en-us/excel
XLSTAT	XLSTAT	https://www.xlstat.com/en/
ImageJ 1.48v	Wayne Rasband (NIH)	https://imagej.nih.gov/ij/