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. 2017 Apr 27;8:504. doi: 10.3389/fimmu.2017.00504

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Copyright © 2017 Chen, Chaurio, Maueröder, Derer, Rauh, Kost, Liu, Mo, Hueber, Bilyy, Herrmann, Zhao and Muñoz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular analysis of the protein-free fraction of supernatants (SNs) from dying cells. Electrograms of SNs of B16F10 cells after 24 h of irradiation and metabolite standards by high-performance liquid chromatography (A). Concentrations of nucleosides measured by mass spectrometry of SNs of B16F10 cells after 24 h of irradiation and 1 h after heat shock (B). Spontaneous adenosine degradation into its metabolites inosine and hypoxanthine in SN spiked with 500 µM of adenosine and measured by mass spectrometry (C).