Fig. 4.

Simple Neurite Tracer is a reliable tool to reconstruct astrocyte process arbors. a Representative GFAP–DAPI staining micrograph (max projection) under 60× magnification, showing detailed astrocytic morphology; scale bar 50 µm. b Representative images of 3D astrocytic morphology reconstruction obtained from different users with SNT. c Similar morphometric results (astrocytic total length, number of processes and process volume) were obtained from different users’ 3D astrocytic reconstructions. d Sholl analysis shows similarity of process arbor complexity of reconstructed astrocytes from different SNT users. e Representative GFAP⁺ cell micrograph (max projection) and its respective reconstruction obtained from AutoNeuron and SNT; scale bar 50 µm. f Astrocytic reconstructions obtained from AutoNeuron and SNT confirm similarity between total length and number of processes. g Sholl analysis confirms similarity between reconstructed astrocytic morphologies at a detailed level of arbor complexity