Fig. 2.

Effect of the PML-RARα fusion protein on mRNA levels of TF, TNF, and IL-β in U-937-PR9 cells. U937-PR9 cells, in which PML-RARα production is under control of the metallothionin promoter, were incubated for up to 48 h with 100 μM of Zn²⁺ to stimulate the production of the PML-RARα fusion protein. Levels of mRNA for TF (left), TNF (center), and IL-1β (right) were quantified by qPCR and expressed relative to that of the corresponding mRNA in control (untreated cells, incubated 24 h in medium alone) U937-PR9 cells. The results are the means ± SEM of at least four independent experiments