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. 2017 Mar 24;409(14):3633–3643. doi: 10.1007/s00216-017-0305-y

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Illustration of the CE-DAD method used for determination of pK _a value of 4-HC in the presence of 4-sulfo-calix[4]arene; the inset graphics show the UV-vis spectra of the acidic (protonated) and basic (deprotonated) analyte forms; 270 and 300 nm are the wavelengths selected to determine variation of β value (see Eq. 9)