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. 2017 Apr 27;8:263. doi: 10.3389/fphys.2017.00263

Figure 2.

Figure 2

Suppression of IL-8 induction by M-CSE or Non-M-CSE in HBECs caused by knockdown or knockout of TRPM8. (A) Cells were incubated with or without TRPM8 siRNA for 24 h. (B) Cells were pretreated with 50 nM TRPM8 siRNA or scramble siRNA for 24 h and then exposed to medium alone, 1% Non-M-CSE or 1% M-CSE for 24 h. (C) Cells were transfected with control plasmid (control) or TRPM8 Double Nickase plasmid (KO). (D) Cells were transfected with control plasmid or TRPM8 KO for 48 h and then exposed to medium alone, 1% Non-M-CSE, or 1% M-CSE for 24 h. Protein levels of TRPM8 or IL-8 were analyzed by Western blot in all panels. mRNA levels of TRPM8 were analyzed by RT-PCR in (C). Data from each group are means ± SEM from four independent experiments. *p < 0.05 vs. the medium group (A,B,D); @p < 0.05 vs. the Non-M-CSE group without pretreatment (B,D); #p < 0.05 vs. the same type of CSE group without pretreatment (B,D).