Table 1.
Peak | Rt (min) | Identity | Absorption spectra (nm) | Peak Purity (%) | Composition (μg/g)* | Identification |
---|---|---|---|---|---|---|
1 | 2.5 | Pheophytin a′ | 652, 468 | 99.3 | 14.6 ± 0.2 | Minguez-Mosquera et al., 1992 |
2 | 4.5 | Pheophytin b | 656, 436 | 98.4 | 1.86 ± 0.5 | Minguez-Mosquera et al., 1992 |
3 | 5.4 | Pheophytin b′ | 654, 440 | 99.5 | 37.2 ± 0.1 | Minguez-Mosquera et al., 1992 |
4 | 6.2 | Pheophytin a | 666, 610 | 99.8 | 20.4 ± 0.3 | Minguez-Mosquera et al., 1992 |
5 | 8.6 | β-Carotene-5,6-epoxide | 470, 442, 418 | 99.8 | 43.6 ± 1.2 | Zeb, 2012 |
6 | 9.3 | 9′-Z-Neoxanthin | 466, 436, 414 | 98.8 | 142.8 ± 1.1 | Updike and Schwartz, 2003 |
7 | 9.9 | All-E-violaxanthin | 470, 440, 416 | 99.6 | 82.2 ± 1.1 | Standard |
8 | 10.4 | Neochrome | 448, 422, 398, | 99.8 | 13.4 ± 0.1 | Edelenbos et al., 2001 |
9 | 10.7 | Neochrome isomer | 448, 422, 398, 280 | 99.9 | 27.2 ± 0.8 | Edelenbos et al., 2001 |
10 | 14.3 | All-E-Lutein | 474, 446, 422 | 99.9 | 806.0 ± 0.5 | Standard |
11 | 16.5 | 9-Z-Lutein | 468, 440, 418, 330 | 99.2 | 51.4 ± 0.5 | Standard |
12 | 17.1 | 9′-Z-Lutein | 466, 440, 418, 330 | 99.3 | 38.8 ± 1.2 | Standard |
13 | 22.1 | Chlorophyll b′ | 652, 464 | 99.5 | 410.0 ± 2.2 | Edelenbos et al., 2001 |
14 | 25.1 | Chlorophyll a | 664, 432 | 99.9 | 162.4 ± 1.1 | Standard |
The compounds were identified by comparing absorption spectra of the sample with the available standards or from the absorption spectra reported in the literature.
Values are expressed as mean ± standard deviation (SD) of replicate readings based on fresh weight.