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. 2017 Apr 26;108(4):795–803. doi: 10.1111/cas.13181

Figure 3.

Figure 3

Knockdown of cofilin‐1 decreases the chemotaxis in PANC‐1 and Capan‐1 pancreatic ductal adenocarcinoma cells. (a) Expression of cofilin‐1 in PANC‐1 and Capan‐1 cells using control siRNA (GL2) or cofilin‐1 siRNA, as determined by Western blot analysis. GAPDH was used as a control for internal protein loading. Cofilin‐1 siRNA dose‐dependently inhibits cofilin‐1 protein expression in PANC‐1 and Capan‐1 cells. (b) Proliferation assay of PANC‐1 and Capan‐1 cells using GL2 or cofilin‐1 siRNA at 3 days after siRNA transfection. (c) Chemotaxis assay of two cell lines using a Boyden chamber. Four randomly selected fields were photographed and the number of migrated cells on the back of the filter was counted. *P < 0.05, **P < 0.01, control siRNA versus cofilin‐1 siRNA. N.S., not significant.