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. 2017 Apr 20;108(4):612–619. doi: 10.1111/cas.13174

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Identification of novel direct target genes of β‐catenin/TCF7L2. (a) Strategy for the identification of candidate target genes of β‐catenin/TCF7L2 complex. (b) Relative expression of the 11 candidate genes in HCT116 (left) and SW480 (right) cells treated with β‐catenin siRNA (gray and black boxes) compared to the cells treated with control siRNA (open boxes). Quantitative PCR was performed in triplicate (mean ± SD) and result was normalized by GAPDH. An asterisk indicates statistical significance (Dunnett's test, P < 0.05) between β‐catenin siRNA and control siRNA.