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. 2017 Apr 20;108(4):641–652. doi: 10.1111/cas.13183

Figure 4.

Figure 4

Dimethyladenosine transferase 1 homolog 1 (DIMT1) regulates myeloma cell survival. (a) Quantitative RT‐PCR for DIMT1 in MM.1S, H929, and KMS11 cells transiently transduced with siDIMT1#1, siDIMT1#2, and control scrambled siRNA (Scr) for 24 h. Bars are mean ± 95% confidence interval of three independent experiments. ***P < 0.001. Student's t‐test was used to test for significance. (b) Western blot analysis of DIMT1, interferon regulatory factor 4 (IRF4), MYC, and Bcl‐2 in MM.1S, H929, and KMS11 cells transiently transduced with siDIMT1#1, siDIMT1#2, and control siRNA (Scr) for 48 h. (c,d) Cell cycle and percentage (%) of cells in sub‐G1, G0/1, S, and G2/M, examined in siDIMT1 transduced multiple myeloma (MM) cells. (e,f) Apoptosis assay in MM cells transiently transduced with siDIMT1#1, siDIMT1#2, and control siRNA (Scr). (e) Annexin V assay. R1, viable (no‐apoptotic) cells; R2, early apoptosis; R3, late apoptosis; R4, necrosis. x‐axis: annexin V; y‐axis: propidium iodide (PI). (f) Percentage (%) of viable cells (R1) and apoptotic cells (R2 and R3). Bars represent mean ± 95% confidence interval of three independent experiments. **P < 0.01; ***P < 0.001. Student's t‐test was used to test for significance. (g,h). In vivo transplantation of shDIMT1 (shDIMT1#1‐GFP, shDIMT1#2‐GFP) or Control‐GFP stably transduced MM.1S cells into NOG mice (n = 10 each). (g) Photograph of tumors. (h) Tumor growth curve. x‐axis, time after transplantation (days); y‐axis, tumor volume (mm3, major × minor2/2). ***P < 0.001. Student's t‐test was used to test for significance. NS, not significant.