Fig. 4.

Methysticin treatment did not significantly prevent amyloid-beta deposition in APP/Psen1 hippocampus. To visualize and quantify amyloid-beta deposition, Congo Red and immunohistochemical staining with a specific antibody against Aβ1-42 were conducted. The analyses were focused on the hippocampus. (A) Amyloid-beta plaque density in this area was analyzed by scanning the hippocampus at 20× magnification. Single pictures were merged using the BZ-II Analyzer Software (Keyence, Neu-Isenburg, Germany). Representative pictures (n=6) of wild type (1st column), untreated APP/Psen1 (2nd column) and methysticin treated (3rd column) hippocampi are shown. The first row represents the pictures of Congo Red staining and the second row the immunohistochemical staining against Aβ1-42. (B) Aβ content in the hippocampus of investigated animals was determined by ELISA. Hippocampal tissue was processed as described in the methods section and data are expressed as pg/µg total protein. (one-way ANOVA with Tukey's post hoc test). Data represent mean+SEM; n=6; *** p<0.001 vs. WT, n.d. not detectable.