Table 1. Primer sequences, annealing temperatures, and predicted product sizes.
Gene | Forward primer | Reverse primer | Tm (°C) | Size (bp) |
β-actin | GCTCTATCCTGGCCTCACTC | GGGTGAGGGACTTCCTGTAA | 55 | 400 |
Collagen I | ACAAGCGATTACACACCCAA | TTAGTTTCCTGCCTCTGCCT | 55 | 239 |
Sp1 | CTCAAAGGAACAGAGTGGCA | GAGCTGGGAGTCAAGGTAGC | 55 | 486 |
Tm: Temperature.