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. 2017 Mar 31;9(4):31. doi: 10.3390/cancers9040031

Figure 2.

Figure 2

NG2 and α3β1 integrin co-localization on U251 glioma cells. NG2-transfected U251 cells were double immunolabeled with rabbit anti-NG2 (A,C,E,G) and mouse monoclonal antibody against either β1 (B,F) or α3 (D,H) integrin. (A–D) U251 cells expressing the T2314E NG2 variant. NG2 and α3β1 co-localize to microprotrusions from the apical cell surface. (E–H) U251 cells expressing the T2256E NG2 variant. NG2 and α3β1 co-localize to prominent lamellipodia on the leading cell edge. Bar in (D) = 5 μm.