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. 2017 Apr 13;2017:2184040. doi: 10.1155/2017/2184040

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Copyright © 2017 Takashi Kitano et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a–d) Histological observation of reepithelialization of skin wound in wild type (WT) ((a1)–(a3), b) and inducible nitric oxide synthase (iNOS-) knockout (KO) ((c1)–(c3), (d1)–(d3)) mice at day 3 ((a1)–(a3), (c1)–(c3)) and day 6 (b, (d1)–(d3)) after injury. Masson-Goldner trichrome stain was viewed at low and high magnification to measure the original wound distance and reepithelialization area, respectively. (e) The ratio of reepithelialization was evaluated. Reepithelialization was significantly delayed in KO mice at days 6 and 8 with statistical difference compared with WT mice. ◆: WT, ▲: KO (days 3, 11, and 14: n = 6; days 6, 8: n = 7 animals in each group). Mean ± standard deviation. ^∗p < 0.05; ^∗∗p < 0.01; bar, 1 mm (a1, b, c1, and d1), 200 μm ((a2)-(a3), (c2)-(c3), and (d2)-(d3)).