Figure 3.

In vitro binding and refolding activities of GroELs and GroES from M. xanthus DK1622. (A) GroEL1 (left) and GroEL2 (right) proteins bound by the His-tag GroES-containing nickel column. Bound GroEL-GroES complexes were eluted from the column with 500 mM imidazole and assayed by SDS-PAGE. Line 1, GroEL1 and GroEL2 proteins that passed through the His-tag GroES column; lines 2–6, increased His-tag GroES bound by the nickel column (0-4 mg) with the same amounts of GroEL proteins for each line; line 7, GroES control with no GroEL. (B) ATPase activities of GroELs in the absence or presence of GroES. For statistical analysis, ^***P < 0.001 and ^**P < 0.01, respectively. (C) MDH renaturation activities of GroELs in the absence or presence of GroES. Error bars in the pictures represent standard deviation of three time repeats.