Table 1. Comparison of BMSC manufacturing methods used by the 8 Centers.

Center	Donor Type	Marrow Source	Density Gradient Separation	Culture Media	Vessels	Number of Passages
1	Healthy Subjects	Marrow Aspirate	No	DMEM* 5% Human Platelet Lysate 2 mM GlutaMax 10 mM N-acetyl cysteine 2 IU/mL heparin	Quantum Bioreactor	2
2	Healthy Subjects	Marrow Aspirate	No	Alpha MEM# 10% FBS+ 5 ng/mL bFGF GlutaMax	T-Flasks	3
3	Healthy Subjects	Marrow Aspirate	Yes	IMDM* 10% “hBM MSC” Supplement^	T-Flasks	4
4	Healthy Subjects	Cryopreserved Marrow Aspirate	No	Alpha MEM 10% FBS+ Glutamax	T-Flasks	3
5	Healthy Subjects	Marrow Aspirate	Yes	Alpha MEM 16.5% FBS+ 1xGlutaMax	T-Flasks and Multiple Layer Flasks	2
6	Healthy Subjects	Marrow Aspirate	No	Alpha MEM with UltraGlutamine 20% FBS+	T-Flasks and Multiple Layer Flasks	4
7	Healthy Subjects and Organ Donors	Fresh or Cryopreserved Marrow Aspirate	Yes and No	Alpha MEM 10% FBS+ GlutaMax	T-Flasks	2 to 3
8	Healthy Subjects	Marrow Aspirate	Yes	Alpha MEM 10% FBS+ GlutaMax	T-Flasks and Multiple Layer Flasks	5

*Dulbecco’s Modified Eagle Medium.

^#Minimum Essential Medium.

*Iscove’s Modified Dulbecco’s Media.

^Stem Cell Technologies.

⁺FBS was not heat-inactivated.