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. 2017 Apr 26;16:84. doi: 10.1186/s12943-017-0653-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — TEX19 is required for cancer cell proliferation and self-renewal: a Left hand line plot: siRNA-mediated (siRNA A) TEX19 mRNA depletion results in inhibition of SW480 cancer cell proliferation (* ≤ 0.05; unpaired t-test); right hand bar chart: RT-qPCR analysis of TEX19 mRNA levels for the cell cultures shown in the adjacent cell count plot (** ≤ 0.01; unpaired t-test; siRNA treatment occurred every 24 h and RNA extraction for RT-qPCR was taken 24 h following final siRNA treatment). b Extreme limiting dilution assay shows that TEX19 siRNA treatment (siRNA A) results in loss of self-renewal capacity in SW480 cancer cells (*** ≤ 0.001; chi square test). Sphere formation was monitored following 10 days incubation. c Left hand line plot: shRNA-mediated (+dox) TEX19 mRNA depletion results in reduction in proliferative potential of SW480 cancer cells (* ≤ 0.05; unpaired t-test; RNA extraction for RT-qPCR was taken 24 h following final Dox treatment); right hand bar chart: RT-qPCR analysis of TEX19 mRNA levels for cell cultures shown in the adjacent plot (* ≤ 0.05; unpaired t-test). d Left hand line plot: SW480 cell carrying a dox-inducible shRNA cassette generate tumours with reduced volume when the mice are fed with dox (red line) compared with no dox (grey line). P value is derived from a Wilcoxon matched pairs test (2-tailed); right hand bar chart: RT-qPCR analysis of TEX19 mRNA levels within final tumours extracted from mice used in the experimental set shown in the adjacent line plot (* ≤ 0.05; unpaired t-test: RNA was extracted for RT-qPCR analysis immediately following termination and tumour extraction for both +/- Dox)