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. 2017 Apr 26;16:84. doi: 10.1186/s12943-017-0653-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of TEX19 depleted SW480 cells: a TEX19 depleted SW480 cells do not have cleaved caspase-3 indicting there is no measurable apoptosis. b TEX19 depleted SW480 cells (lower image) do not form full spheres and exhibit no staining with β-galactosidase indicating that they are not undergoing lysosome-dependent senescence (upper image shows SW480 spheres in the absence of TEX19 siRNA staining positive for β-galactosidase due to natural senescence of internal cells; bar = 250 μm). c FACS of TEX19 siRNA treated SW480 cell populations (a minimum of 10,000 cells were analyzed in each case). d TEX19 siRNA treated cells have a lower proportion of the population in G2 and a higher proportion in S phase. e Western blot of whole cell extracts of SW480 cells depleted for TEX19 have lower levels of phosphor-histone H3 indicating fewer cells in mitosis. f Western blot and RT-qPCR analysis demonstrating TEX19 depletion (for data shown in a and c-e)