Fig. 4.

TEX19 is predominantly localized to the cytoplasm in normal testis and cancer tissues: a Staining of a human testicular seminiferous tubule indicates that TEX19 (red – bottom left panel) is predominantly located in the basal layer of cells and is mostly cytoplasmic. Co-staining with an anti-vimentin antibody (green – top right), which marks Sertoli cells, reveals that TEX19 staining is adjacent to, but not overlapping with vimentin. Bar = 50 μm. b Nuclear foci are apparent when testis are stained with anti-TEX19 (red; blue = DAPI) antibody. White arrows in the merged image show the localization of the nuclear staining with anti-TEX19 antibodies (red; DAPI = blue). c Co-staining with anti-MAGE-A1 antibodies (spermatogonial cells; green) and anti-TEX19 antibodies (red; blue = DAPI) indicate that TEX19 nuclear foci are associated with spermatagonia. White arrows indicate anti-TEX19 stained nuclear foci. Bar = 10 μm. d Staining of clear margin morphologically normal colon tissue taken from a cancer patient indicates that there is no staining with anti-TEX19 antibody (red; right hand panel; blue = DAPI, left hand panel). Bar = 100 μm. e Staining of matched (to tissue shown in D) colon tumour material shows regions of intense staining with anti-TEX19 antibodies (red; blue = DAPI). Bar = 100 μm. f Enlargement of the colon cancer region of intense anti-TEX19 signal (white box; scale bar is 30 μm) shows that anti-TEX19 staining (red) is mostly cytoplasmic