Fig. 3.

Tropomyosin-related kinase B (TrkB) knockdown disrupts colonization and metastatic efficiency of breast cancer brain metastasis (BBM) xenografts. a Representative bioluminescence imaging (BLI) of intracranially injected BBM1 or BBM1-knockdown (BBM1-KD) cells in NOD-SCID mice. b Survival curve of mice injected intracranially with BBM1 or BBM1-KD cells (****p < 0.0001). c Tumor growth over 35 days (n = 6; *p < 0.05; bars indicate SEM). d Hematoxylin and eosin stained brain sections from mice euthanized 28 days after intracranial injection of BBM1 or BBM1-KD cells: tiled images (×5 magnification). White perforated lines indicate locations of the tumor. e The schema for BBM1 and BBM1-KD co-injection study (top). Representative BLI images of mice that received cardiac injections (bottom). f BLI of brain metastases arising from BBM1 cells in mice that received cardiac injections shown in e (n = 6; ****p < 0.0001; bars indicate SEM). Ren renilla, RFP red fluorescent protein, GFP green fluorescent protein