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. 2017 Apr 26;10:206. doi: 10.1186/s13071-017-2136-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Antigen-specific antibody recognition for denatured Rm39, Rm180, Rm239 and Rm76 antigens. Western blot analysis of the recombinant salivary proteins Rm39, Rm180, Rm239 (a) and Rm76 (b) using pooled sera (1:100) from vaccinated calves for the evaluation of antibody responses to linear epitopes. Abbreviations: Bv, before vaccination; 1wpv, 1 week post-third shot of vaccination; 2wpv, 2 weeks post-third shot of vaccination; cainf, challenge infestation (bovines infested with ticks in adult stage); clinf, challenge infestation (bovines infested with ticks in larvae stage); einf, end of infestation