Table 2.

Characteristics of candidate antigens from the R. microplus transcriptome selected using reverse vaccinology for assessment in an anti-tick vaccine

Transcripts from cDNA libraries (RMallHxN dataset)							Recombinant protein			Full-length sequencee
	Developmental stage	No. ESTs in RmRa	Expected No. ESTs in RmRj	No. ESTs in RmSb	Expected No. ESTs in RmS j	P valuec		Amino acids	MWd (kDa)		Amino acids	MW (kDa)
Rm39	All stages	21	30	44	34	0.033f	Rm39	54	5.8	Rm39	105	11
Rm180	Female, Nymph, Male	5	14	27	18	< 0.001g	Rm180	67	7.9	Rm180	186	20.8
Rm239	Nymph, Male	7	18	26	14	0.007h	Rm239	145	16.5	Rm239	500	55

^a R. microplus ticks fed on resistant hosts

^b R. microplus ticks fed on susceptible hosts

^cChi-square test using SigmaPlot 11.0 software

^dMolecular weight calculated without a histidine tag. The size of tagged recombinant proteins is shown in Additional file 2: Figure S1

^eComplete sequences of antigens

^fChi-square test, χ ² = 4.531, df = 1

^gChi-square test, χ ² = 10.864, df = 1

^hChi-square test, χ ² = 7.343, df = 1

^jExpected counts were obtained from software report