Table 1.
Primers used for the amplification and fusion of the katX2 gene with the spectinomycin resistance gene and the flanking homologous sequences
| Name | Sequence (5′–3′) | |
|---|---|---|
| 1 | katA up600 fw | GCGGTGTTCCTGAAAAATAA |
| 2 | katA up600 rev | TGAATTTGTCATGTTATCACCTCTTGGAATTTATAAGAAC |
| 3 | katX2 fw | AGAGGTGATAACATGACAAATTCAAATCATAAAAATTTG |
| 4 | katX2 rev | TTGTTAATTAAATCAATTATTTCATGTTTCCTTGAAGGTAT |
| 5 | spec FW | GAAACATGAAATAATTGATTTAATTAACAACTATGGATATAAAATAG |
| 6 | spec rev | TGCATTTCTCCATTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTT |
| 7 | katA down600 fw | TAAAAAAATTATAATAATGGAGAAATGCAAAAACC |
| 8 | katA down600 rev | TTAAAAGGGAAAAGTTCTCATAGC |